polyclonal rabbit anti prongf  (Alomone Labs)

Journal: Neurobiology of disease

Article Title: Cholinotrophic basal forebrain system alterations in 3xTg-AD transgenic mice.

doi: 10.1016/j.nbd.2010.10.002

Figure Lengend Snippet: Fig. 6. (A) Representative immunoblot from the cortex of 2 to 3, 13 to 15 and 18 to 20 months old 3xTg-AD and ntg probed with antibodies against proNGF and β-actin. (B and C) Linear representation of mean densitometry measurements of cortical (B) and hippocampal (C) proNGF-ir signals normalized to β-actin-ir in 2 to 3, 13 to 15 and 18 to 20 months old 3xTg-AD and age-matched ntg mice. Statistical analysis revealed higher levels of proNGF in the cortex of aged 3xTg-AD mice compared to middle-aged and young mutant mice, but unchanged levels in ntg mice. In addition, hippocampal normalized proNGF-ir levels were significantly increased in both aged 3xTg-AD and ntg mice compared to their respective middle-aged and young 3xTg-AD and ntg mice (*p=b0.001, data were analyzed with a Kruskal–Wallis non-parametric test followed by Dunn's post hoc test for multiple comparisons). Abbreviations: ntg, non-transgenic mice; 3xTg, 3xTg-AD transgenic mice; error bars=standard error of the mean; mo, months.

Article Snippet: Rabbit polyclonal antibodies against proNGF (H-20, 1:50, Santa Cruz, CA, USA), p75NTR (1:300; Abcam, Cambridge, MA) and TrkA (1:50; Fitzgerald Industries International, Inc, MA) were used for quantitative immunoblotting.

Techniques: Western Blot, Mutagenesis, Transgenic Assay